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Genome Engineering and Synthetic Biology are revolutionizing Life Sciences. Driven by advances in the CRISPR-toolbox for rapid, cheap, multiplex modification of genomes and breakthroughs in DNA synthesis technologies, the pace of progress enabled by these tools in the last years has been breathtaking. The first three editions (September 2013, January 2016, January 2018) were a roaring success and we are pleased to announce the 4th edition of GESB in beautiful Ghent in September, 2021.